We are generating high quality, annotated assemblies for many. These cultivars were selected by the project principal investigators to represent genetic diversity typical of breeding programs in their agroecological zones.

We are using a two pronged approach (see image below). For eight cultivars, we are completing RefSeq assemblies using NRGene’s DeNovoMagicV3.0 assembler. For the remaining cultivars, we are generating assemblies using the open access W2RAP assembly algorithms developed at the Earlham Institute to specifically assemble complex genome from short read sequences .  All assemblies generated will be joined into chromosomal sequence using a combination of 10X Genomics data and Hi-C. 


De Novo Assembly Australia Langridge, U of A Mace Yuma//PI 372129/3/CO850034/4/4*Yuma/5/(KS91H184/Arlin S//KS91HW29/3/NE89526)
De Novo Assembly Australia Langridge, U of A Lancer VI184/Chara//Chara/3/Lang
De Novo Assembly Canada C. Pozniak, P. Hucl U of S, Andrew Sharpe -GIFS CDC Stanley CDC Teal//EE8/Kenyon35//AC Barrie
De Novo Assembly Canada C. Pozniak, P. Hucl U of S, Andrew Sharpe -GIFS CDC Landmark Unity/BW864 (BW864 is Alsen/Superb)
De Novo Assembly Germany N. Stein, K. Meyer IPK Julius Julius = Asketis x Drifter
De Novo Assembly Japan K. K. Shimizu, YCU Norin61 Fukuoka komugi 18/Shinchunaga
De Novo Assembly Switzerland B. Keller, U of Zurich S. Krattinger - King Abdullah University ArinaLrFor _
De Novo Assembly USA Poland, K State Jagger KS-82-W-418/STEPHENS
Assembly WR2AP UK BBSRC Cadenza Axona x Tonic, RBP95-73, Maris Dove, HPG-522-66
Assembly W2RAP UK BBSRC Paragon CSW-1724-19-5-69 x Axona/Tonic, introgression
of diccocoides on 5B
Assembly W2RAP UK BBSRC Kronos _
Assembly W2RAP UK BBSRC Robigus 1366 x Z-836
Assembly W2RAP UK BBSRC Claire Flame x Wasp,/Moulin/Taurus/Boxer/Galahad